fab4841a  (R&D Systems)

Journal: Journal of the American Society of Nephrology

Article Title: The IL-23/Th17 Axis Contributes to Renal Injury in Experimental Glomerulonephritis

doi: 10.1681/asn.2008050556

Figure Lengend Snippet: Figure 1. Detection of renal Th17 cells in experimental glomer- ulonephritis. (A) Representative intracellular cytokine staining as- sessed by flow cytometry for production of IL-17 in renal T cells 10 d after induction of nephritis. T cells were isolated from the kidneys of nephritic mice (NTN) or controls and cultured with or without PMA (5 ng/ml)/ionomycin (1 g/ml) for 5 h. Dot plots show intracellular IL-17 staining of CD3 T cells. (B) Intracellular IL-17/IFN double staining of renal T cells from nephritic mice. Results are representative of three independent experiments.

Article Snippet: For T cell differentiation, renal single-cell suspension was stained with fluorochrome-labeled antibodies specific for CD3 (APC; 17A2, R&D Systems, Wiesbaden, Germany) and CD4 (PE; GK1.5, Miltenyi, Ber- gisch Gladbach, Germany) for 25 min at 4°C.

Techniques: Staining, Flow Cytometry, Isolation, Cell Culture, Double Staining